Fig. 4

Rearing under different larval densities produced distinct wing shapes in adult Brazilian Ae. aegypti females. Scatterplot comparisons of wing shape for (a) wMel_Br and (b) wMel_BrTET female mosquitoes reared at either low (green dots), intermediate (blue dots) or high (red dots) larval densities, based on analysis of the main canonical variates predicting wing shape pattern. These data are depicted without allometry. Each circle represents a single adult female. Shape variations are depicted with the aid of thin-plate spline deformation grids (c) for PC1, PC2 and PC3, since 62.3 % of the observed variation was concentrated in these three principal components. Shape variation was scaled down 10 times in this analysis since it over-exaggerates the observed variation. Data were pooled from two independent biological replicates