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Table 1 Design of a study to determine the efficacy and speed of kill of DPP against adult R. sanguineus s.l

From: Efficacy and speed of kill of a topically applied formulation of dinotefuran-permethrin-pyriproxyfen against weekly tick infestations with Rhipicephalus sanguineus (sensu lato) on dogs

Day Procedure
-14 to -7 Acclimatization to kennel environment
-7 Infestation of all dogs with 50 adult ticks
-5 Tick counts and allocation of dogs to three groups of eight
-2 Infestation of dogs with 50 adult ticks
0 Topical application of DPP to dogs in Groups 2 and 3; in situ tick counts on dogs in Groups 1 and 3 at 2 and 8 h after treatment
1 Removal of ticks from dogs in Group 3 and withdrawal of this group of dogs from the study
2 Tick counts and tick removal on dogs in Groups 1 and 2
7, 14, 21, 28, 35, 42 Infestation of dogs in Groups 1 and 2 with 50 adult ticks; in situ tick counts on dogs in Groups 1 and 2 at 2 and 8 h after infestation
9, 16, 23, 30, 37, 44 Tick counts and tick removal on dogs in Groups 1 and 2, 48 h after infestation