Day | Procedure |
---|---|
-14 to -7 | Acclimatization to kennel environment |
-7 | Infestation of all dogs with 50 adult ticks |
-5 | Tick counts and allocation of dogs to three groups of eight |
-2 | Infestation of dogs with 50 adult ticks |
0 | Topical application of DPP to dogs in Groups 2 and 3; in situ tick counts on dogs in Groups 1 and 3 at 2 and 8 h after treatment |
1 | Removal of ticks from dogs in Group 3 and withdrawal of this group of dogs from the study |
2 | Tick counts and tick removal on dogs in Groups 1 and 2 |
7, 14, 21, 28, 35, 42 | Infestation of dogs in Groups 1 and 2 with 50 adult ticks; in situ tick counts on dogs in Groups 1 and 2 at 2 and 8 h after infestation |
9, 16, 23, 30, 37, 44 | Tick counts and tick removal on dogs in Groups 1 and 2, 48 h after infestation |