Table 3 List of primers used in SYBR Green qRT-PCR
Gene | Sequence (5′ to 3′) | |
|---|---|---|
Homo sapiens COL1A1 | Forward | CATGTTCAGCTTTGTGGACCTC |
(NM_000088.3) | Reverse | TCACAGATCACGTCATCGCA |
Homo sapiens COL3A1 | Forward | CTTCTCTCCAGCCGAGCTTC |
(NM_000090.3) | Reverse | GTAGTCTCACAGCCTTGCGT |
Homo sapiens TβRII | Forward | GTAGCTCTGATGAGTGCAATGAC |
(NM_003242.5) | Reverse | CAGATATGGCAACTCCCAGTG |
Homo sapiens α-SMA | Forward | CAGCCAAGCACTGTCAGGAAT |
(NM_001613.2) | Reverse | TTTGCTCTGTGCTTCGTCAC |
Homo sapiens β-actin | Forward | TGGCACCCAGCACAATGAA |
(NM_001101.3) | Reverse | CTAAGTCATAGTCCGCCTAGAAGCA |