Fig. 2
LD RPA assay analytical sensitivity. a Fluorescence development in one RPA run by using 107–101 DNA molecules/reaction of the LD DNA molecular standards (Graph generated by ESEquanttubescanner studio software). The sensitivity was 100 DNA molecules. No fluorescence was recorded between 3 and 4 min because a mixing step is necessary to increase the assay sensitivity. b Reproducibility of LD RPA assay using data sets of eight RPA assay runs using the DNA molecular standards. LD RPA assay produced results between 3 and 12 min. 107–102 DNA molecules were detected 8 out of 8 runs. 101 copies were not identified by the LD RPA assay. The error bars represent the standard deviation. No error bars were shown for 106–104 because values were consistence at 5.7 min in all eight RPA runs. c The probit regression analysis using data of eight RPA assay runs. The limit of detection at 95 % probability (39 DNA molecules) is depicted by a triangle