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Table 3 Stability and adsorption characteristics of NATOG. Concentrations were obtained after spiking. Samples were analyzed in duplicate. Accuracy of 80–120 % on the theoretical spiked concentration was considered acceptable

From: Evaluation of the diagnostic potential of urinary N-Acetyltyramine-O,β-glucuronide (NATOG) as diagnostic biomarker for Onchocerca volvulus infection

  NATOG μM
Stabilitya Ref. 24 h 4 °C 24 h RT 24 h 50 °C
Urine pH 4 11.0 ± 0.3 10.7 ± 0.0 11.0 ± 0.4 10.9 ± 0.0
Urine pH 6 10.9 ± 0.3 11.2 ± 0.1 11.4 ± 0.0 11.7 ± 0.1
Urine pH 8 11.6 ± 0.3 11.6 ± 0.1 11.6 ± 0.2 11.8 ± 0.1
Sun stabilityb Ref. 0.5 h Suntest 3 h Suntest  
Clear vials 2.65 ± 0.01 2.61 ± 0.03 2.57 ± 0.01  
Amber vials 2.64 ± 0.01 2.65 ± 0.01 2.61 ± 0.03  
Adsorption testa Ref. glass 6 cycles glass Ref. PP 6 cycles PP
Urine pH 4 10.5 ± 0.3 11.0 ± 0.1 10.7 ± 0.1 10.8 ± 0.1
Urine pH 6 11.2 ± 0.1 11.1 ± 0.1 10.7 ± 0.8 11.1 ± 0.1
Urine pH 8 11.3 ± 0.1 11.4 ± 0.1 11.5 ± 0.1 11.3 ± 0.0
  1. aUrine with an endogenous NATOG concentration of 5.13 μM was spiked with 5.63 μM NATOG
  2. bSample of 2.81 μM NATOG in Milli-Q water
  3. Abbreviations: Ref. reference, RT room temperature, PP Polypropylene