Fig. 5

Sliding window analysis of nucleotide diversity in the ITS marker of (a) Berwaldia and (b) MIC1. A sliding window of 50 base pairs was used, with an increment of 25 base pairs. Observed nucleotide diversity was calculated as the average heterozygosity per site (Tajima’s estimator, π), while the expected nucleotide diversity was computed as the average number of nucleotide differences per site (Watterson’s estimator, θ _w). The gap in positions 350–400 in the MIC1 graph is the result of complete sequence identity, which renders the estimator inapplicable