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Table 1 In vitro activity of Taxodium distichum and the reference drugs ivermectin and diethylcarbamazine (DEC) on adult worms (AW) and microfilariae (Mf) of Brugia malayi assessed by motility assay (MA) and MTT reduction assay

From: Antifilarial activity of diterpenoids from Taxodium distichum

Antifilarial agent LC100 a (μg/ml) for AW in MA IC50 b (μg/ml) for AW in MA Mean % inhibition in MTT reduction by AW LC100 (μg/ml) for Mf in MA IC50 (μg/ml) for Mf in MA CC50 c (μg/ml) SI for AW in MA SI for Mf in MA
A001 (crude extract) 15.63 10.00 47.00 3.91 1.95 250 25 128.29
F001 31.25 9.84 79.63 7.83 4.92 210 21.34 42.68
F002 > 125 17.00 > 125 88
F003 > 125 38.00 > 125 88
F004 > 125 42.00 > 125 88
SF1 31.25 12.40 46.23 7.82 < 3.95 250 20.16 > 63.29
SF2 7.82 5.52 78.08 7.82 < 3.95 430 77.89 > 108.86
SF3 > 250 45.03 250.00 95 340 3.78
SF4 125.00 74.33 83.86 62.50 22.10 330 4 14.93
SF5 > 250 43.31 125
SF6 > 250 32.89 250
K001 62.50 41.23 76.89 250 88.38 697 16.91 7.88
K002 > 250 > 250 38.05 > 250 > 250
K003 125 74.33 82.05 31.25 18.58 180 2.42 9.69
K004 125 52.56 85.02 31.25 11.05 190 3.61 17.19
Ivermectin (μM) 5 3.05 5.80 2.5 1.57 250 81.96 159.23
DEC-C (μM) 1000 314.98 62.54 500 297.30 9000 28.57 30.27
  1. aLC100 = 100 % reduction in motility indicates death of parasite
  2. bIC50 = concentration of the agent at which 50 % inhibition in motility of the parasites is achieved
  3. cCC50 = concentration at which 50 % of cells are killed
  4. Abbreviations: SI Selectivity Index (CC50/IC50), DEC-C Diethylcarbamazine-citrate
  5. Motility score of parasite in Control wells (parasite + DMSO only) = 4 (i.e. parasites were motile and highly active); MTT absorbance values (OD510nm) of control wells (parasites + DMSO only) = 0.64