Table 1 In vitro activity of Taxodium distichum and the reference drugs ivermectin and diethylcarbamazine (DEC) on adult worms (AW) and microfilariae (Mf) of Brugia malayi assessed by motility assay (MA) and MTT reduction assay
From: Antifilarial activity of diterpenoids from Taxodium distichum
Antifilarial agent | LC100 a (μg/ml) for AW in MA | IC50 b (μg/ml) for AW in MA | Mean % inhibition in MTT reduction by AW | LC100 (μg/ml) for Mf in MA | IC50 (μg/ml) for Mf in MA | CC50 c (μg/ml) | SI for AW in MA | SI for Mf in MA |
|---|---|---|---|---|---|---|---|---|
A001 (crude extract) | 15.63 | 10.00 | 47.00 | 3.91 | 1.95 | 250 | 25 | 128.29 |
F001 | 31.25 | 9.84 | 79.63 | 7.83 | 4.92 | 210 | 21.34 | 42.68 |
F002 | > 125 | – | 17.00 | > 125 | 88 | – | – | – |
F003 | > 125 | – | 38.00 | > 125 | 88 | – | – | – |
F004 | > 125 | – | 42.00 | > 125 | 88 | – | – | – |
SF1 | 31.25 | 12.40 | 46.23 | 7.82 | <Â 3.95 | 250 | 20.16 | >Â 63.29 |
SF2 | 7.82 | 5.52 | 78.08 | 7.82 | <Â 3.95 | 430 | 77.89 | >Â 108.86 |
SF3 | > 250 | – | 45.03 | 250.00 | 95 | 340 | – | 3.78 |
SF4 | 125.00 | 74.33 | 83.86 | 62.50 | 22.10 | 330 | 4 | 14.93 |
SF5 | > 250 | – | 43.31 | 125 | – | – | – | – |
SF6 | > 250 | – | 32.89 | 250 | – | – | – | – |
K001 | 62.50 | 41.23 | 76.89 | 250 | 88.38 | 697 | 16.91 | 7.88 |
K002 | > 250 | > 250 | 38.05 | > 250 | > 250 | – | – | – |
K003 | 125 | 74.33 | 82.05 | 31.25 | 18.58 | 180 | 2.42 | 9.69 |
K004 | 125 | 52.56 | 85.02 | 31.25 | 11.05 | 190 | 3.61 | 17.19 |
Ivermectin (μM) | 5 | 3.05 | 5.80 | 2.5 | 1.57 | 250 | 81.96 | 159.23 |
DEC-C (μM) | 1000 | 314.98 | 62.54 | 500 | 297.30 | 9000 | 28.57 | 30.27 |