Genetic dynamics in the sand fly (Diptera: Psychodidae) nuclear and mitochondrial genotypes: evidence for vector adaptation at the border of Iran with Iraq

Table 4 Tajima’s D neutrality analyses and neutral allele frequency test (Fu’s F _s) of the Cyt b and EF-1α gene regions in Phlebotomus and Sergentomyia species

Species	P. papatasi	P. alexandri	Subgenus Sergentomyia	Subgenus Sintonius
Genes	Cyt b	Cyt b	Cyt b	EF-1α	Cyt b
Fragment length (bp)	788	789	789	497	789
m	6	5	7	6	6
S	431	428	367	276	35
p _s	0.555	0.560	0.682	0.584	0.0445
Θ	0.243	0.269	0.278	0.256	0.0296
π	0.332	0.335	0.375	0.305	0.0296
D	2.391	1.876	2.041	1.250	0
A-T ratio (%)	70.92	73.84	68.4	46.82	70.02
G-C ratio (%)	29.08	26.16	31.6	53.18	29.98
G	3.632	7.265	1.11	2	0.05
K ₁	13.762	2.88	54.065	12.325	13.039
K ₂	9.879	3.198	60.416	1.391	20.427
Fu’s F _s	3.047	3.568	Sergentomyia genus		-0.463
			0.913	1.482

m = number of sequences, S = number of segregating sites, p _s = S/m, Θ = p _s/a₁, π = nucleotide diversity, D is the Tajima statistic test, G = the discrete Gamma distribution, K ₁ and K ₂ are the transition/transversion bias rate ratios of purines and pyrimidines respectively

ISSN: 1756-3305