Molecular detection of Bartonella spp. in terrestrial leeches (Haemadipsa rjukjuana) feeding on human and animal blood in Gageo-do, Republic of Korea

Kang, Jun-Gu; Won, Sohyun; Kim, Hye-Won; Kim, Baek-Jun; Park, Bae-Keun; Park, Tae-Seo; Seo, Hong-Yul; Chae, Joon-Seok

doi:10.1186/s13071-016-1613-3

Table 1 Nucleotide sequences of polymerase chain reaction primers and conditions used for amplification of Bartonella spp. and host genes from land leeches

From: Molecular detection of Bartonella spp. in terrestrial leeches (Haemadipsa rjukjuana) feeding on human and animal blood in Gageo-do, Republic of Korea

Target gene	PCR	Name of the PCR primer used	Sequence (5′–3′)	Annealing temperature	Amplicon size (bp)	Reference
Bartonella spp. ITS	1^st	QHVE1	TTCAGATGATGATCCCAAGC	58	735	[21]
	1^st	QHVE4	AACATGTCTGAATATATCTTC	58	735
	2^nd	QHVE12	CCGGAGGGCTTGTAGCTCAG	58	484
	2^nd	QHVE14	CACAATTTCAATAGAAC	58	484
Anaplasma spp.16S rRNA	1^st	AE1-F	AAGCTTAACACATGCAAGTCGAA	56	1,406	[21]
Anaplasma spp.16S rRNA	1^st	AE1-R	AGTCACTGACCCAACCTTAAATG	56	1,406
Anaplasma phagocytophilum 16S rRNA	2^nd	EE3	GTCGAACGGATTATTTTTATAGCTTGC	56	926
Anaplasma phagocytophilum 16S rRNA	2^nd	EE4	CCCTTCCGTTAAGAAGGATCTAATCTCC	56	926
Anaplasma bovis 16S rRNA	2^nd	ABkf	TAGCTTGCTATGGGGACAA	59	547
Anaplasma bovis 16S rRNA	2^nd	ABlr	TCTCCCGCACTCCAGTCTG	59	547
Ehrlichia spp. 16S rRNA	1^st	ECC	AGAACGAACGCTGGCGGCAAGC	56	450	[22]
Ehrlichia spp. 16S rRNA	1^st	ECB	CGTATTACCGCGGCGCTGGCA	56	450
Ehrlichia chaffeensis 16S rRNA	2^nd	HE3	TATAGGTACCGTACTTATCTTCCCTAT	56	390
Ehrlichia chaffeensis 16S rRNA	2^nd	HE1	CAATTGCTTATAACCCTTTTGGTTATAAAT	56	390
Ehrlichia canis 16S rRNA	2^nd	HE3	TATAGGTACCGTACTTATCTTCCCTAT	56	365
Ehrlichia canis 16S rRNA	2^nd	ECAN5	CAATTATTTATAGCCTCTGGCTATAGGC	56	365
Borrelia burgdoferi 16S rRNA	1^st	B1	CAGTGCGTCTTAAGCATGC	59	1,427	[21]
	1^st	B8	CCTTAAATACCTTCTCCC	59	1,427
	2^nd	B3	GCAGCTAAGAATCTTCCGCAATGG	60	714
	2^nd	B6	CAACCATGCAGCACTGTATAT	60	714
Rickettsia sp. gltA	1^st	RpCS. 877p	GGGGACCTGCTCACGGCGG	54	382	[23]
	1^st	RpCS. 1258n	ATTGCAAAAAGTACAGTGAA	54	382
	2^nd	RpCS. 896p	GGCTAATGAAGCAGTGATAA	58	338
	2^nd	RpCS. 1233n	GCGACGGTATACCCATAG	58	338
Wolbachia 16S rRNA		16S-F	TTGTAGCCTGCTATGGTATAACT	56	937	[24]
Wolbachia 16S rRNA		16S-R	GAATAGGAGTTTTCATGT	56	937	[24]
mt DNA cytochrome b gene		L14841	AAAAAGCTTCCATCCAACATCTCAGCATGATG	50	450	[25]
mt DNA cytochrome b gene		H15149	AAACTGCAGCCCCTCAGAATGATATTTGTCCTCA	50	450	[25]

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ISSN: 1756-3305

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