Leishmania from | CL | ML | DL |
---|
IGF-I (50 ng/ml) | – | + | – | + | – | + |
Parasite isolates | 124 | 107 | 139 | 199 | 255 | 180 |
78 | 87 | 146 | 148 | 110 | 133 |
449 | 455 | 73 | 73 | 33 | 33 |
140 | 145 | 177 | 182 | 36 | 28 |
135 | 161 | 108 | 126 | 122 | 125 |
412 | 365 | 38 | 45 | 33 | 34 |
85 | 106 | 106 | 136 | 38 | 30 |
153 | 133 | | | | |
- THP-1 cells were infected with the isolates of L. (V.) braziliensis from patients with cutaneous (CL, n = 8), mucosal (ML, n = 7) or disseminated (DL, n = 7) forms of the disease and stimulated or not with IGF-I (50 ng/ml) throughout the culture period of 48 h. Parasitism was evaluated by optical microscopy and the result is represented as the number of parasites per 100 cells. Results representative of two experiments with each isolate tested in triplicate