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Fig. 2 | Parasites & Vectors

Fig. 2

From: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Fig. 2

a N. caninum-based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH 3–10 NL, IPG strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules (arrow). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars: 0.5 μm

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Parasites & Vectors

ISSN: 1756-3305

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