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Fig. 3 | Parasites & Vectors

Fig. 3

From: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Fig. 3

mRNA levels of NcGRA7 and NcNTPase. Real time-PCR was employed to quantify the mRNA transcription of both proteins along the lytic cycle. a Photomicrographs showing the infection dynamics of the Nc-Liv isolate on MARC-145 cultures at recent invasion (6 hpi), PV maturation (24 hpi), exponential growth of parasites (48 hpi) and tachyzoite egress (56 hpi and 48 hpi + DTT). Scale-bars: 20 μm. b mRNA levels of NcGRA7 and NcNTPase during the lytic cycle. c Effect of DTT supplementation to artificially induce egress at 48 hpi on mRNA levels for both proteins. For a and b, each point represents a single sample and bars represent the mean value. a, b and c indicate significant differences (Kruskal-Wallis test, followed by Dunn’s test)

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Parasites & Vectors

ISSN: 1756-3305

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