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Fig. 4 | Parasites & Vectors

Fig. 4

From: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Fig. 4

Confocal laser scanning microscopy of NcNTPase along the lytic cycle of tachyzoites. Infected cultures were fixed with methanol (MeOH), paraformaldehyde (PFA) and paraformaldehyde combined with glutaraldehyde (PFA + GA). Then, coverslips were double labelled with affinity purified antibodies against NcNTPase (red) and monoclonal antibodies against NcSAG1 (green). Nuclei were stained with DAPI (blue). All the images show a single 1 μm slice. Scale-bars: 4 μm

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