Fig. 4From: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egressConfocal laser scanning microscopy of NcNTPase along the lytic cycle of tachyzoites. Infected cultures were fixed with methanol (MeOH), paraformaldehyde (PFA) and paraformaldehyde combined with glutaraldehyde (PFA + GA). Then, coverslips were double labelled with affinity purified antibodies against NcNTPase (red) and monoclonal antibodies against NcSAG1 (green). Nuclei were stained with DAPI (blue). All the images show a single 1 μm slice. Scale-bars: 4 μmBack to article page