Skip to main content
Fig. 5 | Parasites & Vectors

Fig. 5

From: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Fig. 5

a Confocal laser scanning microscopy of NcGRA7 along the lytic cycle of tachyzoites. Infected cultures were fixed with methanol (MeOH), paraformaldehyde (PFA) and paraformaldehyde combined with glutaraldehyde (PFA + GA). Then, coverslips were double labelled with affinity purified antibodies against NcGRA7 (red) and monoclonal antibodies against NcSAG1 (green). Nuclei were stained with DAPI (blue). b Confocal laser scanning microscopy of NcNTPase (green) and NcGRA7 (red) along the lytic cycle of tachyzoites. Methanol-fixed cultures were double labelled with affinity purified antibodies against NcNTPase (green) and monoclonal antibodies against NcGRA7 (red). Images taken at 1 hpi, 24 hpi and 56 hpi show a single tachyzoite, a parasitophorous vacuole and a large group of tachyzoites undergoing egress, respectively. Nuclei were also stained with DAPI (blue). All the images show a single 1 μm slice. Scale-bars: 4 μm

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us