Fig. 1

Structural property and phylogenetic position of C. sinensis omega GST1 and 2 (CsGSTo). a Comparison of primary structure of CsGSTo1 and 2 with other related members. Residues directly contacting glutathione are indicated by asterisks. Glutathione-binding residues are marked by closed circles. Cysteine residues that constitute the active site of omega GSTs are denoted by red letters. Putative thioredoxin and GST_C domains are indicated by dotted green- and red-boxes. Dots represent gaps introduced into the sequences to optimize sequence identities. CsGSTo1, Clonorchis sinensis omega GST1 (KX163088); CsGSTo2, C. sinensis omega GST2 (KX163089); SmGSTO, Schistosoma mansoni omega GST (AAO49385); FhGSTO, Fasciola hepatica omega GST (JX156880); EgSspA, Echinococcus granulosus stringent starvation protein A (CDJ25309); HmSspA: Hymenolepis microstoma stringent starvation protein A (CDJ10775); CeGSTO-1, Caenorhabditis elegans omega GST (GAA34234); HsGSTO1 and 2, Homo sapiens omega GST1 and 2 (AAF73376 and AAH56918). Gene names are adapted from the GenBank database (http://www.ncbi.nlm.nih.gov/). b Comparison of genomic structure of CsGSTo1 and 2 with platyhelminth and human orthologues. Coding DNA sequences are presented with solid squares in proportion to their relative sizes. The 5′- and 3′-untranslated regions are marked with open squares (voluntary length). Intervening introns are shown by solid lines (fixed length). Numerals in parentheses indicate the phase of each intron. The lengths of exons and introns in bp are presented. The dotted boxes show exons, which have acquired introns during evolution of paralogous/orthologous genes. c Phylogenetic relationships of CsGSTos. The phylogenetic position of CsGSTos was predicted on the basis of alignment of amino acid sequences. The tree was constructed by the neighbor-joining algorithm of PHYLIP. Numbers at the major branching nodes demonstrate their percentages of appearance in 1,000 bootstrap replicates. GenBank accession numbers of missing entities in a include FgGSTO, Fasciola gigantica omega GST (AFX98105); EmSspA, Echinococcus multilocularis stringent starvation protein A (EmuJ_000919600); CeGSTO-2, Caenorhabditis elegans omega GST-2 (CCD62560); CeGSTO-3, C. elegans omega GST-3 (CCD72880); OvGSTO, Onchocerca volvulus omega GST (AAF99575); BmGSTO1, Bombyx mory omega GST1 (NP_001040131); BmGSTO2, B. mory omega GST2 (NP_001037406); BmGSTO3, B. mory omega GST3 (NP_001040435); BmGSTO4, B. mory omega GST4 (NP_001108461); DmGstO1, Drosophila melanogaster omega GST-1 (NP_648237); DmSepia, D. melanogaster Sepia (NP_648235); DmGstO2A, D. melanogaster omega GST2A (NP_729388); DmGstO2B, D. melanogaster omega GST2B (NP_648236); DmGstO3, D. melanogaster omega GST3 (NP_648235)