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Fig. 2 | Parasites & Vectors

Fig. 2

From: Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress

Fig. 2

Binding affinity of the native CsGSTos toward S-hexylglutathione (SHG) and reduced glutathione (GSH). a C. sinensis adult extracts (200 μg) bound each with SHG-bead and glutathione-Sepharose 4B were eluted using 4 mM SGH or 4 mM GSH. The bound proteins (100 ng) were separated by 12 % reducing SDS-PAGE, transferred to nitrocellulose membranes and probed with anti-rCsGSTo1 and 2. The membranes were developed with ECL. r1 and r2, rCsGSTo1 and 2 (each 100 ng) loaded as positive controls. UB, unbound fractions of C. sinensis extracts; W, washing fractions; Eluent, bound fractions eluted with 0, 2 and 4 mM SHG or GSH. b 2-DE profile of native CsGSTo1 and 2. The bound proteins of SHG-agarose bead (10 μg) were isoelectrically focused using IPG strip (pH 3–10), after which resolved by 12 % SDS-PAGE and blotted onto nitrocellulose membrane. The membranes were incubated with anti-rCsGSTo1 and 2 antibodies (1:1,000 dilution) and subsequently with HRP-conjugated goat anti- mouse IgG (1:4,000 dilution). The blots were developed with ECL

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