Fig. 5From: Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stressImmunolocalization of C. sinensis GSTo1 and compartmental expression of C. sinensis GSTo1 and 2 transcripts. a Adult worm sections (thickness 4-μm) were incubated with preimmune mouse serum (panels 1) or anti-rCsGST1 antibody (panels 2) at a same dilution ratio (1:200) and subsequently incubated with HRP-conjugated goat anti-mouse IgG antibody (1:1,000 dilutions). The slides were developed with the blue-immunohistochemistry chromogen 3,3'-diaminobenzidine blue supplemented with H2O2. Acetocarmine-stained in toto specimen is also seen. The regions marked by dotted-ellipses were separately prepared as vitelline follicle-enriched parenchyma for Western blot (see also Fig. 7). Abbreviations: OS, oral sucker; IN, intestine; VS, ventral sucker; VF, vitelline follicles; SR, seminal receptacle; TE, testis. Scale-bars: 50 μm. b Expression of CsGSTo transcripts in C. sinensis reproductive system by qRT-PCR. Total RNAs (each 200 ng) extracted from respective organs were reverse transcribed into cDNA and subjected to qRT-PCR. The fold increase was calculated by differences in threshold cycles (ΔΔCT). Key: 1, egg; 2, whole worm; 3, testis, seminal receptacle and vitelline follicle-enriched fractions; 4, parenchymal fractions without reproductive organsBack to article page