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Fig. 6 | Parasites & Vectors

Fig. 6

From: Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress

Fig. 6

Induction profiles of C. sinensis GSTo transcripts and proteins under oxidative stress conditions. a The fresh live adults (10 worms per group) were stabilized in serum-free RPMI media for 1 h at 37 °C and were treated with Juglone (25–100 μM) or cumene hydroperoxide (CHP; 1–4 mM) for 1 h at 37 °C. Total RNAs (200 ng) were reverse-transcribed and subjected to qRT-PCR. The fold increase was calculated by differences in threshold cycles (ΔΔCT). b The worms were incubated in the presence of Juglone (100 μM) or CHP (4 mM) from 15–60 min as indicated. Total RNAs (200 ng) were extracted and fold increase (ΔΔCT) of each transcript was examined by qRT-PCR. *P < 0.05; **P < 0.01. c, d Proteins (20 μg) extracted from respective compartments the parasites incubated with different doses of CHP (1–4 mM) and different time intervals (15–60 min) were separated by 12 % reducing SDS-PAGE, blotted to nitrocellulose membranes and probed with anti-rCsGSTo1 and 2, respectively. C. sinensis tubulin, which did not show expressional changes upon oxidative stimuli, was used as a control. Positive signals were detected with ECL. Vitelline follicle-enriched fractions were prepared from the bilateral margins of middle portions of the worm, as marked in Fig. 5

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