Fig. 7
Protective roles of CsGSTos in E. coli transfected with CsGSTo expression plasmids under oxidative stress. a Disc diffusion assays using CsGFSTos overexpressing E. coli. LB agar was overlaid with top agar containing 5 × 108 E. coli cells transfected with recombinant CsGSTo plasmids or mock vector. Filter-discs soaked with 10, 50, 100 and 200 mM of cumene hydroperoxide (CHP) or Juglone were placed on the plate and incubated overnight, after which the inhibition zones (halo diameter) were measured. *P < 0.05; **P < 0.01. b Effect of CHP (upper panel) or Juglone (lower panel) on the survival of exponentially growing E. coli. Cells were incubated for 60 min at 37 °C in the presence of 2 mM CHP or Juglone. c Survival curves of E. coli following exposure to different doses of CHP or Juglone (1, 2 and 4 mM) for 20 min at 37 °C. *P < 0.05; **P < 0.01. Data shown represent mean ± standard deviation, SD (n = 3)