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Table 1 Substrate specificity of recombinant CsGSTo1 and 2

From: Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress

  Substrate Class-specificity Specific activity (μmol/min/mg)
rGSTo1 rGSTo2
GST-specific CDNBa All 0.13 ± 0.01 0.10 ± 0.02
  Cumene hydroperoxide α ndb nd
  DCNBc μ nd nd
  Ethacrynic acid π nd nd
  4-nitrobenzyl chloride μ and θ 1.42 ± 0.09 0.91 ± 0.24
  4-nitrophenyl acetate ω 0.84 ± 0.06 0.73 ± 0.04
  4-hydroxy nonenal α and θ nd nd
DHARd Dehydroascorbate ω 1.16 ± 0.04 1.08 ± 0.02
Thioltransferase Hydroxyethyl disulfide ω 1.08 ± 0.02 0.76 ± 0.02
  1. a1-chloro-2,4-dinitrobenzene
  2. bNot detected
  3. c1,2-dichloro-4-nitrobenzene
  4. dDehydroascorbate reductase