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Table 1 Substrate specificity of recombinant CsGSTo1 and 2

From: Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress

 

Substrate

Class-specificity

Specific activity (μmol/min/mg)

rGSTo1

rGSTo2

GST-specific

CDNBa

All

0.13 ± 0.01

0.10 ± 0.02

 

Cumene hydroperoxide

α

ndb

nd

 

DCNBc

μ

nd

nd

 

Ethacrynic acid

Ï€

nd

nd

 

4-nitrobenzyl chloride

μ and θ

1.42 ± 0.09

0.91 ± 0.24

 

4-nitrophenyl acetate

ω

0.84 ± 0.06

0.73 ± 0.04

 

4-hydroxy nonenal

α and θ

nd

nd

DHARd

Dehydroascorbate

ω

1.16 ± 0.04

1.08 ± 0.02

Thioltransferase

Hydroxyethyl disulfide

ω

1.08 ± 0.02

0.76 ± 0.02

  1. a1-chloro-2,4-dinitrobenzene
  2. bNot detected
  3. c1,2-dichloro-4-nitrobenzene
  4. dDehydroascorbate reductase