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Table 2 Kinetic parameters for recombinant CsGSTo1 and 2

From: Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress

Kinetic parameter

rCsGSTo1

rCsGSTo2

DHA

Vmax

1.16 ± 0.02 μmol/min/mg

1.08 ± 0.02 μmol/min/mg

 

Km

0.21 ± 0.02 mM

0.17 ± 0.02 mM

 

Kcat

0.48 s

0.43 s

 

Kcat/Km

2.29 × 103/s/M

2.53 × 103/s/M

GSH

Vmax

0.42 ± 0.04 μmol/min/mg

0.56 ± 0.08 μmol/min/mg

 

Km

0.19 ± 0.02 mM

0.16 ± 0.02 mM

 

Kcat

0.37 s

0.33 s

 

Kcat/Km

1.95 × 103/s/M

2.06 × 103/s/M

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Parasites & Vectors

ISSN: 1756-3305

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