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Fig. 4 | Parasites & Vectors

Fig. 4

From: Microsatellite marker analysis of Haemonchus contortus populations from Pakistan suggests that frequent benzimidazole drug treatment does not result in a reduction of overall genetic diversity

Fig. 4

Median joining network of 89 H. contortus isotype-1 β-tubulin sequences from seven populations (Pop24S, Pop1S, Pop27G, Pop2G, Pop3S, Pop5G and Pop13S). A full median network containing all possible shortest trees was generated by setting the epsilon parameter equal to the greatest weighted distance (epsilon = 10) using Network 4.6.1 (Fluxus Technology Ltd.). All unnecessary median vectors and links are removed with the MP option [19]. The size of circle representing each haplotype is proportional its frequency in the overall dataset and the colours in the circles reflect the frequency distribution in each geographical location as indicated on the colour key on the inset map. Small red dots represent median vectors. The number of mutations separating adjacent sequence nodes or median vectors is indicated along connecting branches and the length of the lines connecting haplotypes is proportional to the number of nucleotide changes. The most probable ancestral node is determined by rooting the network to a closely related outgroup H. placei (Hp) (GenBank accession number KJ598498.1). The text providing the name of each haplotype is colour-coded as follows; susceptible haplotype F200Y (TTC)/F167Y (TTC)/E198A (GAA) is in black text; P200Y resistant haplotype F200Y (TAC)/F167Y (TTC)/E198A (GAA) is in blue text

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