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Table 1 Primers used for Borrelia-specific 16S rRNA and flaB genes amplification in this study, including primer sequences, annealing temperature and expected product size

From: Novel Borrelia species detected in echidna ticks, Bothriocroton concolor, in Australia

Gene

Primer

Sequence (5' – 3')

Annealing temperature

Expected product size (bp)

Reference

16S

External

    
 

Bor-16 F

TGCGTCTTAAGCATGCAAGT

   
 

Bor-1360R

GTACAAGGCCCGAGAACGTA

51 °C

1,344

This study

 

Internal

    
 

Bor-27 F

CATGCAAGTCAAACGGAATG

   
 

Bor-1232R

ACTGTTTCGCTTCGCTTTGT

51 °C

1,205

This study

flaB

External

    
 

FlaB280F

GCAGTTCARTCAGGTAACGG

   
 

FlaRL

GCAATCATAGCCATTGCAGATTGT

52 °C

645

[33, 66]

 

Internal

    
 

flaB_737F

GCATCAACTGTRGTTGTAACATTAACAGG

   
 

FlaLL

ACATATTCAGATGCAGACAGAGGT

55 °C

407

[33, 66]