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Fig. 2 | Parasites & Vectors

Fig. 2

From: Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population

Fig. 2

Silencing of the Ae. aegypti (RJ strain) VGSC results in increased susceptibility to a pyrethroid insecticide. a Mortality percentage in Ae. aegypti adults from RJ strain (after dsRNA treatment in larval stage) after 30 min of exposure to deltamethrin. Third-instar larvae were soaked in water (untreated) or in dsRNA corresponding to the VGSC (VGSC422 dsRNA), P-glycoprotein (PgP dsRNA) and P450 enzymes (CYP9J26, CYP9J32). Data are shown as the mean ± standard deviation of four replicates. Statistics: One-way Anova, followed by Tukey’s multiple comparisons test. b Comparative VGSC gene expression analysis between different developmental stages of Rock and RJ strains. Data are shown as the mean ± SD of 6 replicates. Statistics: Multiple t-test. c Ae. aegypti RJ strain larvae were soaked as described in a, but using three different concentrations of VGSC422, as indicated. Statistics: One-way Anova followed by Tukey’s multiple comparison test. d VGSC gene expression level was determined by quantitative RT-PCR (qRT-PCR) and performed on untreated or dsRNA-VGSC422 treated (0.5 μg/μL) Ae. aegypti RJ 4th instar larvae. Data are shown as the mean ± SD of 6 experiments performed in triplicate. Statistics: t-test. e VGSC gene expression level was determined by qRT-PCR and performed on untreated or dsRNA-VGSC422 treated (0.5 μg/μl) Ae. aegypti RJ adults. Data are shown as the mean ± SD of 8 experiments performed in triplicate. Statistics: t-test. *P < 0.01, ***P < 0.0001; ****P < 0.00001

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