Fig. 5
RNAi-effect optimization by refining the dsRNA constructs for the VGSC gene. a Schematic representation of 6 transcript variants of VGSC gene and the 8 overlapping dsRNA sequences used to silence the gene (Tile 1 to Tile 8). Deletion sites in variant RB and RD of variant AAEL006019 are indicated by a dotted line, as well as the position of mutation Val1016Ile and Phe1534Cys sites. b Mortality percentage of 5 day-old Ae. aegypti RJ adult females after 30 min exposure to deltamethrin. The adult females were treated at the 3rd larval stage by different dsRNA-VGSC tiles. Data show the mean ± standard deviation of three replicates from 3 independent experiments performed. Statistics: One-way Anova followed by Tukey’s multiple comparisons test. c Mortality percentage of 5 day-old Ae. aegypti RJ adult females after 30 min exposure to deltamethrin. The adult females were treated at the 3rd larval stage by three different concentrations of Random or VGSCT6 dsRNAs. Data are shown as the mean ± standard deviation of three replicates (2 independent experiments were performed). Statistics: One-way Anova followed by Tukey’s multiple comparisons test. d VGSC gene expression level was determined by qRT-PCR and performed on dsRNA-Random or dsRNA-VGSC422 treated and pyrethroid-affected Ae. aegypti RJ adults. Statistics: t-test *P < 0.05, ****P < 0.00001