Fig. 6From: Immunomodulatory effects of tick saliva on dermal cells exposed to Borrelia burgdorferi, the agent of Lyme diseaseTLR2 and IL-10RA qRT-PCR results THP-1 RNA was extracted from 2-h cultures stimulated with B. burgdorferi (1:10) alone, B. burgdorferi and saliva or B. burgdorferi and 0.7 μM PC. Real-time PCR was performed for TLR2 and IL-10RA relative expression levels. a, Transcript levels from cells exposed to B. burgdorferi and saliva showed a significant decrease in TLR2 expression at 2 h post-stimulation (***P = 0.001); b, Transcript levels from cells exposed to B. burgdorferi and saliva showed a significant increase in IL-10RA expression (***P = 0.015)Back to article page