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Fig. 2 | Parasites & Vectors

Fig. 2

From: Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis

Fig. 2

ESA antibodies were tested by ELISA and immunoblotting analyses. a Levels of ESA antibodies after each immunization. Two groups of 2 female BALB/c mice were immunized five times at 2-week intervals. PBS was used to immunize 2 mice in the control group. Blood sera were sampled from the tails of mice 10 days after immunization. Each sample was measured in duplicate. Values represent the mean and standard deviation. b Western blotting analysis of ESA. Lane 1: marker on gel; Lane 2: ESA on gel; Lane 3: marker on NC membrane; Lane 4: ESA on NC membrane. The membranes were coloured with 3, 3’-diaminobenzidine

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