Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis

Xue, Junxin; Jiang, Wei; Chen, Yongjun; Liu, Yingchun; Zhang, Huajing; Xiao, Yan; Qiao, Yuanbiao; Huang, Kehe; Wang, Quan

doi:10.1186/s13071-016-1643-x

Table 2 CAg proteins identified by LC-MS/MS after IP enrichment and purification with ESA antibodies

From: Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis

Resource ID	Uniprot Database ID	Identified protein name	Peptide count	Unique peptide count^a	Sequence coverage^b (%)	Theoretical Mr (Da)/pI^c	References of analysis in T. gondii	Functional clustering^d
Micronemes
1	D8L558	MIC4	4	4	8.62	63,061/5.05	[36, 37]	Protein metabolic process
2	S8F3P1	MIC1	3	3	4.17	48,628/5.15	[37]	Binding
3	T2FG71	MIC3	2	2	7.81	35,165/5.97	[38]	Unknown
Dense granules
4	P13403	GRA1	4	3	25.79	20,149/4.18	[39]	Extracellular region
5	S7UP40	GRA5	2	2	13.33	12,976/5.78	[40]	Extracellular region
Surface
6	S7V2Y4	SAG1	4	4	15.77	34,828/8.34	[41]	Membrane
7	I7CQQ5	SAG2	2	2	19.18	14,814/6.17	[41]	Unknown
Novel CAg proteins
8	S7VXR0	RPL40	3	3	26.36	14,685/9.84	na	Protein metabolic process
9	S8ETG3	Coronin	3	3	3.70	68,441/5.5	na	Protein metabolic process
10	S8GEU1	ELMO	2	2	0.71	243,844/9.91	na	Phagocytosis
11	S8GDY4	Uncharacterized protein	2	2	0.51	295,608/6.23	na	Unknown
12	V4Z4M8	Chorein	2	2	0.11	1,451,666/9.02	na	Unknown
13	V4ZB05	Putative transmembrane protein	2	2	0.99	211,754/4.91	na	Unknown
14	V4ZM84	Uncharacterized protein	2	2	0.65	235,435/5.95	na	Unknown
15	V4YZ80	Ubiquitin-transferase	2	2	0.09	1,711,013/6.68	na	Protein metabolic process
Other
16	S7VTE3	Protein disulfide isomerase	13	12	29.72	52,801/5.14	[42]	Protein metabolic process
17	P90613	Lactate dehydrogenase	14	11	41.34	35,548/6.03	[43, 44]	Oxidation-reduction process
18	S7VNC9	Actin	9	7	24.39	32,058/5.66	[45]	Binding
19	S8GUS9	ACT1	3	2	4.52	41,907/5.05	[45]	Binding
20	S7W8N1	Chaperonin protein BiP (HSP70)	4	4	5.54	73,252/5.23	[46]	Response to stress
21	S8G950	Enolase 2	3	3	10.53	52,113/6.43	[47]	Carbohydrate metabolic process
22	B9Q0N7	14-3-3 protein	2	2	4.64	37,173/5.05	[48]	Binding
23	S7URH5	Fructose-bisphosphate aldolase	11	11	34.41	46,959/9.01	[49]	Carbohydrate metabolic process
24	S7WD35	Phosphofructokinase PFKII	3	3	3.43	131,361/5.85	[50]	Carbohydrate metabolic process
25	Q2HXR1	Phosphorylase family protein	2	2	8.58	33,042/6.66	[51]	Nucleoside metabolic process
26	S8GL48	Type I fatty acid synthase	2	2	0.21	1,093,457/5.96	[52]	Oxidation-reduction process

^aResults include proteins that have a unique peptide count ≥ 2
^bSequence coverage is defined as the ratio (%) of matched residues in the entire sequence
^cTheoretical Mr/pI was obtained from Uniprot database
^dThe functions are classified by gene ontology (GO) annotations
Abbreviation: na, not available

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ISSN: 1756-3305

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