Fig. 2

Subcellular localization of expressed mosquito odorant receptors in HEK293 cells. a1 HEK293 cells (no fluorescence). a2 Same as a1 with AalOR7 expression of AalOR7-eGFP. a3 Same as a1 with AalOR7-eGFP-transfected cells stained with DID cell-labelling solution. a4 Same as A1 with AalOR7 expressed in HEK293 cells stained with DID membrane stain. b1 AalORs transcripts detected by RT-PCR in HEK293 cells 24 h after transfection. Lane M: molecular weight marker in the 2,000 bp series; Lane 1: AalOR7; Lane 2: AalOR10; Lane 3: AalOR88. b2 HEK293 cells expressing AalOR7-eGFP. b3 HEK293 cells expressing AalOR10-DsRed. b4 HEK293 cells co-expressing AalOR7-eGFP and AalOR10-DsRed