Golden hamster gene target | Primer sequences | Reference | Amplicon length | Slope | Intercept | Coefficient of linearity (r2) | Amplification efficiency (%) |
---|---|---|---|---|---|---|---|
GAPDH | Fw 5′-GGT TGC CAA ACC TTA TCA GAA ATG-3′ | Ribeiro-Romão et al. [11] | 194 bp | -3.30 | 20.03 | 0.98 | 100.7 |
Rv 5′-TTC ACC TGT TCC ACA GCC TTG-3′ | |||||||
ɣ Actin | Fw 5′-ACA GAG AGA AGA TGA CGC AGA TAA TG-3′ | Espitia et al. [18] | 70 bp | -3,28 | 20.06 | 0.99 | 101.7 |
Rv 5′-GCC TGA ATG GCC ACG TAC A-3′ | |||||||
IFN-ɣ | Fw 5′-TGT TGC TCT GCC TCA CTC AGG-3′ | Espitia et al. [18] | 130 bp | -3.48 | 26.03 | 0.99 | 93.4 |
Rv 5′-AAG ACG AGG TCC CCT CCA TTC-3′ | |||||||
TGF-β | Fw 5′-GGC TAC CAC GCC AAC TTC TG-3′ | Espitia et al. [18] | 81 bp | -3.52 | 27.11 | 0.99 | 92.3 |
Rv 5′-GAG GGC AAG GAC CTT ACT GTA CTG-3′ | |||||||
TNF | Fw 5′-TGA GCC ATC GTG CCA ATG-3′ | Espitia et al. [18] | 79 bp | -3.33 | 24.81 | 0.99 | 99.5 |
Rv 5′-AGC CCG TCT GCT GGT ATC AC-3′ | |||||||
IL-10 | Fw 5′-GGT TGC CAA ACC TTA TCA GAA ATG-3′ | Espitia et al. [18] | 194 bp | -3.21 | 29.66 | 0.99 | 104.7 |
Rv 5′-TTC ACC TGT TCC ACA GCC TTG-3′ | |||||||
IL-4 | Fw 5′-CCA CGG AGA AAG ACC TCA TCT G-3′ | Zivcec et al. [25] | 72 bp | -2.98 | 31.06 | 0.98 | 116.4 |
Rv 5′-GGG TCA CCT CAT GTT GGA AAT AAA-3′ | |||||||
IL-6 | Fw 5′-GGA CAA TGA CTA TGT GTT GTT AGA A-3′ | This study | 99 bp | -3.23 | 28.85 | 0.97 | 103.8 |
Rv 5′- AGG CAA ATT TCC CAA TTG TAT CCA G-3′ | |||||||
iNOS | Fw 5′-TGA GCC ACT GAG TTC TCC TAA GG-3′ | Osorio et al. [26] | 93 bp | -3.54 | 29.71 | 0.95 | 92.7 |
Rv 5′-TCC TAT TTC AAC TCC AAG ATG TTC TG-3′ | |||||||
Arginase | Fw 5′-ACC TAT GTG TCA TTT GGG TGG A-3′ | Osorio et al. [26] | 163 bp | -3.38 | 20.57 | 0.99 | 97.5 |
Rv 5′-GCA GAT ATG CAG GGA GTC ACC-3′ |