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Table 1 Ranges of parasite burden of Trypanosoma cruzi determined by real-time PCR in samples of Triatoma infestans used in xenodiagnosis (qPCR-XD) and peripheral blood (qPCR-B) in 100 individuals with chronic Chagas disease classified by positive or negative XD

From: Quantification by real-time PCR of Trypanosoma cruzi DNA in samples of Triatoma infestans used in xenodiagnosis of chronic Chagas disease patients

  Positive XDa Negative XDb
  n = 21 n = 79
Ranges parasite burden (parasite equivalent/ml) qPCR-XD†c qPCR-B qPCR-XD†c qPCR-B
  n (%) n (%) n (%) n (%)
No Ct 0 (0) 0 (0) 13 (16.5) 17 (21.5)
0.1–1 0 (0) 8 (38.1) 27 (34.2) 29 (36.7)
1–10 2 (9.5) 7 (33.3) 32 (40.5) 30 (38.0)
10–100 5 (23.8) 4 (19.0) 5 (6.3) 2 (2.5)
100–1,000 9 (42.9) 1 (4.8) 2 (2.5) 1 (1.3)
1,000–10,000 5 (23.8) 1 (4.8) 0 (0) 0 (0)
  1. †Heteroscedasticity detected: Levene's test, F = 187.663, P = 0.0001
  2. aChi-square test (qPCR-XD vs qPCR-B): χ 2 = 91.82, df = 5, P = 0.0001
  3. bChi-square test (qPCR-XD vs qPCR-B): χ 2 = 6.71, df = 5, P = 0.1520
  4. cKruskal-Wallis test: χ 2 = 42.48, df = 2, P = 0.0001
  5. Abbreviation: No Ct, No threshold cycle (DNA-T.cruzi was not detected)