Fig. 1

Diagrams of the putative amino acid sequences of PfPK2 and TgCaMKrk, and of the expression plasmids used in this study. a The amino acid sequences of PfPK2 and TgCaMKrk are shown; the catalytic and regulatory domains, including the autoinhibitory and calmodulin-binding domains [27], are shaded. TgCaMKrk has no regulatory domain. The arrow denotes the conserved catalytic lysine. b The plasmids (pEU-GST-TgCaMKrk and pEU-GST-TgCaMKrkKA) used for the expression of GST-TgCaMKrk and GST-TgCaMKrkKA, respectively, in the wheat germ cell-free protein synthesis system are shown. The SP6 promoter (SP6), translational enhancer (E01), GST, and tobacco etch virus protease recognition site are indicated [36]