Fig. 3

In vitro kinase assays of purified GST-GFP, GST-TgCaMKrk, and GST-TgCaMKrkKA. a Purified GST-GFP (Lane 1), GST-TgCaMKrk (Lane 2), or GST-TgCaMKrkKA (Lane 3) was incubated in kinase buffer containing [γ-³²P]ATP, separated on a denaturing gel, and stained with CBB. b Autoradiograph of the gel shown in a. c Purified GST-TgCaMKrk incubated in kinase buffer containing [γ-³²P]ATP (Lane 1) and the labeled protein treated with λ-protein phosphatase (Lane 2) were separated on a denaturing gel and then stained with CBB. d Autoradiograph of the gel shown in c. Molecular masses (kDa) are indicated on the left