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Fig. 5 | Parasites & Vectors

Fig. 5

From: Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog

Fig. 5

Expression of TgCaMKrk in T. gondii-infected cells. a Immunoblotting was performed using lysates from T. gondii (Lanes 1 and 2) or Vero cells (Lanes 3 and 4) and anti-TgCaMKrk mouse serum (Lanes 2 and 4) or pre-immune mouse serum (Lanes 1 and 3). The arrowhead indicates expression of TgCaMKrk. Molecular masses (kDa) are indicated on the left. b Localization of TgCaMKrk in T. gondii. Mouse anti-TgCaMKrk antibody was used as the primary antibody, and anti-GRA6, -GAP45, -ROP1 and -M2AP antibodies from rabbits were used as markers of parasite organelles. Nuclei are stained with TO-PRO3. The four upper panels indicate the extracellular parasite; the four lower panels indicate the intracellular T. gondii. Differential interference contrast (DIC) images and overlaid images are shown in the two right panels. Scale-bar: 5 μm

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