Fig. 5

Comparative growth kinetics of PCV (circles) and WNV (squares) in C6/36 (RNAi-deficient) (a) and RML-12 (RNAi-competent) (b) Aedes albopictus cells. Cells were infected with either PCV or WNV at an MOI of 0.1 and infectious titers at each time-point (up to 5 or 7 days) determined by titration of culture supernatant on C6/36 cells and detection of infected wells by fixed cell ELISA. Error bars represent standard deviation and asterisks indicate significance (P < 0.0001) as determined by a two-way ANOVA