Fig. 2

Effect of EDTA and dose-dependence of Jaburetox-induced in vitro aggregation of hemocytes. Hemolymph was collected, diluted with Rhodnius saline and incubated with different concentrations of Jaburetox alone or Jaburetox plus 100 μM of Na₂EDTA for 1 h at room temperature. Controls were performed employing equivalent volumes of saline. The number of aggregates (a) and free cells (b) was counted using a hemocytometer. The values are expressed as the number of aggregates or the number of free cells per ml of hemolymph. Mean ± SEM (n = 3 for controls and n = 5 for Jaburetox-treated insects). ***P < 0.0001 vs control