Fig. 4

Effect of inhibitors of eicosanoid synthesis on the in vitro Jaburetox-induced hemocyte aggregation. Hemolymph was collected, diluted with Rhodnius saline and incubated with different concentrations of Jaburetox alone or Jaburetox plus the indicated concentrations of dexamethasone (dexa), indomethacin (indo), esculetin (escu) or baicalein (baic) for 1 h at room temperature. Controls were performed employing equivalent volumes of saline and ethanol. The number of aggregates (a) and free cells (b) was counted using a hemocytometer. The values are expressed as the number of aggregates or the number of free cells per ml of hemolymph. Mean ± SEM (n = 4 for controls and n = 6 for Jaburetox-treated insects). ***P < 0.0001 vs control; **P < 0.01 vs control; *P < 0.05 vs control