Fig. 8

Detection of active caspase 3 in cultured hemocytes. Cultured hemocytes incubated with PB (control) (a-c) or with 50 (d-f) and 100 nM (g-i) Jaburetox for 24 h were stained with the anti-cleaved caspase 3 antibody (b, e, h) and counterstained with DAPI (a, d, g). Arrowheads indicate cells positive for cleaved caspase-3 while asterisks point to cells with incipient positive signal for the antibody. The figure shows representative experiments of three independent assays. Scale-bars: 10 μm (magnification of 1000×)