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Table 1 Annotations of the most abundant host proteins detected in Glossina m. morsitans SGs. Proteins are listed from the most abundant in descending order

From: A proteomics approach reveals molecular manipulators of distinct cellular processes in the salivary glands of Glossina m. morsitans in response to Trypanosoma b. brucei infections

UniProt ID Protein name Mol. weight (kDa) Sequence coverage (%) Peptides Log10 iBAQ -Log t-test (P-value) t-test ratios (infected vs unparasitized) Description/functional roles in insects
D3TMW5 Tsetse salivary gland protein 2 43.955 87.9 50 [2] 9.48 2.67 -0.53 (↓) Blood-feeding; immunogenic
D3TLK6 Tsetse salivary gland growth factor-1 56.59 78.9 66 [5] 9.46 3.60 -0.49 (↓) Blood-feeding and anti-haemostasis
D3TQL1 Salivary antigen 5 variant 28.925 78.4 32 [18] 9.36 3.18 -0.69 (↓) Blood-feeding and other extensive physiological roles
Q9NBA5 Tsetse salivary gland protein 1 45.613 87.0 45 [20] 9.21 2.16 -0.58 (↓) Blood-feeding; immunogenic
D3TRV7 5′-nucleotidase-related (5′Nuc) saliva protein 62.064 67.2 37 [37] 8.80 3.06 -0.58 (↓) Blood-feeding; downregulated in parasitized flies
D3TQW4 Adenosine deaminase-related growth factor C 62.2 65.6 39 [32] 8.73 1.96 -0.38 (↓) Cell proliferation; non-immunogenic
Q9NBA4 Tsetse salivary gland protein 2 44.001 84.5 48 [9] 8.71 1.88 -0.52 (↓) Involved in blood-feeding; immunogenic
D3TPT6 Actin 87E 41.831 72.1 25 [1] 8.65 0.21 0.10 (↑) Overexpressed in hytrosavirus-infected tsetse
D3TKU2 Adenine deaminase 24.14 46.7 20 [2] 8.48 3.01 -0.47 (↓) Blood-feeding; vector competence; cellular proliferation
D3TKU0 5′nucleotidase 100.2 27.7 30 [30] 8.45 1.42 -0.44 (↓) Blood-feeding; downregulated in parasite-infected flies
Q9U7C5 Tsetse salivary gland growth factor-2 58.222 57.1 40 [40] 8.40 2.09 -0.54 (↓) Blood-feeding and antihaemostasis; non-immunogenic
D3TR78 Lectin 19.762 63.3 16 [16] 8.39 2.09 -0.36 (↓) Influence trypanosome establishment and maturation
D3TPN5 Arginine kinase 40.029 65.4 26 [25] 8.04 1.05 0.19 (↑) Abundantly expressed in silkworms; insect homeostasis
Q9U7C6 Tsetse salivary gland growth factor-1 56.631 76.3 63 [2] 8.02 0.44 -0.24 (↓) Blood-feeding and antihaemostasis; non-immunogenic
D3TQC9 Muscle LIM protein at 84B 10.077 61.3 6 [1] 7.90 1.46 0.32 (↑) Muscle/epithelia differentiation in Drosophila
D3TQ00 Myosin heavy chain 87.317 67.1 68 [68] 7.79 0.09 0.08 (↑) Overexpressed in hypertrophied tsetse SG
D3TRK1 Trehalose-6-phosphate synthase 31.361 57.2 18 [18] 7.74 2.14 -0.29 (↓) Tsetse housekeeping gene involved in trehalose synthesis
D3TLM8 Multifunctional chaperone (14-3-3-ζ) 28.213 59.7 13 [11] 7.69 2.64 0.36 (↑) Intracellular adaptor in diverse biological processes
D3TRW4 ATP synthase β 54.579 64.3 21 [20] 7.62 0.70 0.20 (↑) Ion transporter
D3TN30 Cytochrome c2 11.768 54.6 7 [7] 7.62 1.13 0.29 (↑) Essential mitochondrial respiratory chain component
D3TR28 Calponin 20.152 81.5 15 [15] 7.60 0.05 0.01 (↑) Ca2+-binding protein; associated with wound-healing
D3TLI1 Troponin I 24.523 36.1 9 [9] 7.52 0.28 0.12 (↑) An actin-binding protein
D3TQ27 Cofilin actin depolymerizing factor/Cofilin) 17.167 73.0 12 [12] 7.47 2.99 0.48 (↑) Control of actin assembly in cells
D3TNV8 Elongation factor 1α 50.403 60.9 18 [13] 7.46 0.79 -0.15 (↓) Translation elongation
D3TQW6 Salivary secreted adenosine 41.221 29.2 20 [2] 7.46 1.13 -1.96 (↓) Non-immunogenic ADGF (also known as ADGF-3)
  1. Except salivary secreted adenosine (significantly downregulated), all the other proteins shown in this table were insignificantly upregulated (n = 10) or downregulated (n = 15) in parasitized SGs compared to the unparasitized GGs. Upregulated and downregulated proteins are indicated by upward (↑) and downward (↓) arrows, respectively. The unique peptides for each of the proteins listed in the table are shown in square brackets in column 5