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Table 2 Annotation of 32 Glossina-specific proteins significantly upregulated in parasitized SG proteome of Glossina m. morsitans compared to the unparasitized SG proteome

From: A proteomics approach reveals molecular manipulators of distinct cellular processes in the salivary glands of Glossina m. morsitans in response to Trypanosoma b. brucei infections

UniProt ID Protein name LC-MS/MS measurements/quantification Protein descriptions/functional annotation
Mol. weight (kDa) Sequence coverage (%) Unique peptides -Log t-test (P-value)
D3TQ33 Ca2+/calmodulin-dependent protein kinase 24.064 58.1 11 5.14 Calcyphosin-like protein; regulation of ion transport
D3TMA1 Tetraspanin 42Ed 25.291 13.5 3 3.98 Acts as scaffold/anchor to specific cell membranes
D3TQS8 Integrin beta-PS 27.471 24.5 5 5.64 IGF-like repeat protein; cell adhesion to extracellular matrix proteins
H9TZT6 Stress-associated ER protein-2 42.284 17.6 4 4.21 ER stress
D3TQD5 Small ubiquitin-related modifier 3 10.328 35.2 3 1.49 Essential regulator of cellular processes (e.g. survival of stressed cells).
D3TMM3 Vacuolar H+−ATPase v0 sector subunit D 39.805 19.1 4 3.60 Cation trans-epithelia transport (SGs, labial glands; midguts; sensory sensilla)
D3TMK9 Chaperonin containing t-complex polypeptide-1ζ 58.314 13.7 5 4.20 TcP-1 family members are involved in the prevention of the aggregation of proteins unfolded by stress, or newly synthesized proteins
D3TM06 Chaperonin containing t-complex polypeptide-1θ 60.02 11.8 5 5.57
D3TLV7 Chaperonin containing t-complex polypeptide-1ζ 59.292 10.9 4 5.46
D3TLP9 Chaperonin containing t-complex polypeptide-1α 59.192 5.0 3 1.21
D3TMQ1 Mitochondrial methylglutaconyl-CoA hydratase 31.879 19.1 4 4.18 Metabolism of branched-chain amino acids (e.g. leucine, isoleucine and valine)
D3TLC7 Isovaleryl-CoA dehydrogenase 46.634 5.7 2 3.84
D3TNK0 Hypothetical conserved protein 37.228 10.8 4 4.70 70 % homologous to D. melanogaster uracil-DNA degrading factor-like protein (UDE); conformational integrity of DNA-protein complex machinery
D3TPX7 α-carboxylesterase αE7 65.575 21.8 11 1.44 Lipid metabolism in insects
D3TMQ8 24-kDa mitochondrial glutamine synthetase 44.028 4.2 2 2.03 Metabolism of glutamate (important product of ammonia detoxification)
D3TLS2 Mitochondrial NADH-ubiquinone oxidoreductase 26.829 13.2 3 3.08 Mitochondrial electron transport/transfer
D3TRJ5 Cytochrome b-c1 complex-7 13.551 28.8 3 0.92
D3TPP1 Downstream of receptor kinase 24.434 13.7 3 3.19 Essential roles in immune responses
D3TMW2 Proteasome subunit beta type-4 30.601 7.7 2 4.76 Associates with polo-like kinase; increase 20S proteasome to proteolytic activity
D3TPR8 Translin 28.855 14.4 3 3.66 Various biological roles (e.g. control and distribution of nucleic acid metabolism)
D3TLJ6 Mitochondrial prohibitin-2 36.64 9.7 3 1.01 Conserved protein involved in biogenesis and maintenance of mitochondria
D3TMK2 Ras-related small GTPase, rho type 21.289 12.0 2 5.47 Molecular switches that govern various important cellular functions
D3TRZ7 Aspartate aminotransferase 45.969 21.9 6 1.10 A key enzyme in amino acid metabolism
D3TRZ8 Gamma-glutamyl hydrolase 43.565 4.5 2 5.92 Folate metabolism
D3TPR2 Myosin essential light chain 16.572 27.2 3 0.93 A structural component of the actomyosin cross-bridge
D3TRA4 Transaldolase 37.217 20.5 5 0.95 Provides a link between glycolytic and pentose phosphate pathways
D3TLJ8 Dihydrolipoamide S-acetyltransferase 55.469 8.2 3 1.14 Provides a link between glycolytic and TCA cycles
D3TNC5 Retrotransposon protein 28.099 17.4 3 2.87 Mobile element that transpose by reverse transcription
D3TP54 Actin-related protein 2/3 complex-3 20.502 15.8 2 3.31 Induction of actin polymerization during pathogen infection
D3TQ55 Hypothetical secreted protein 24.355 38 2 2.60 Homologous (97 %) to salivary secreted mucin; tsetse mouthpart lubricant
Q0QHK6 δ-1-pyrroline-5-carboxylate dehydrogenase 58.318 26.3 10 2.61 Amino acid (glutamate and proline) metabolism
D3TRY4 Cathepsin B-like cysteine proteinase 38.221 14.1 4 3.37 SG cell autophagic cell death