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Fig. 3 | Parasites & Vectors

Fig. 3

From: Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection

Fig. 3

Immunolocalisation of S. scabiei Ssλ20∆B3 and Ssλ15 antigens in skin sections derived from a mange-infested chamois. a, b Peroxidase anti-peroxidase immunostaining after reaction with a rabbit polyclonal anti-Ssλ15 serum, c a rabbit polyclonal anti-Ssλ20∆B3 serum, d a rabbit pre-immune serum. Ssλ15 immunolabeling is located in the tegument around the mouthparts and around the coxa of the legs (a and b), and Ssλ20∆B3 was located in the integument and the spaces surrounding the parasite’s vital organs (c). No staining is observed in mites and host tissues when using pre-immune sera, confirming that the detected immunolabeling is specific (d). Abbreviations: A, anterior end of mite; P, posterior end of mite; Sp, spicules; M, mouthparts; L, legs; SB, stomach blocks; IT, the integument of the epidermis; Sy, synganglion; Mu, striated muscle. Scale-bars: a, b, 50 μm; c, d, 20 μm

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Parasites & Vectors

ISSN: 1756-3305

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