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Fig. 6 | Parasites & Vectors

Fig. 6

From: Saliva of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) inhibits classical and alternative complement pathways

Fig. 6

Ability of Rhipicephalus microplus saliva to displace complement alternative pathway factors already deposited. Bb = factor Bb; P = properdin. Human serum was added to a microplate pre-coated with agarose, leading to the formation of C3 convertase. After 30 min, R. microplus saliva was added and the presence of complement component deposits was evaluated using anti-C3, anti-factor B and anti-properdin polyclonal antibodies. Bars represent the arithmetic mean ± standard deviation (SD) from three biological replicates. Statistically significant differences between samples with and without saliva were assessed using t-test (C3b t (2) = 1.31, P = 0.32; Bb t (2) = 1.99, P = 0.18; P t (2) = 3.33, P = 0.09). Abbreviation: ns, non-significant (P > 0.05)

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