Table 2 Interactions of CpROMs, TgROM2 and MIC proteins by yeast two-hybrid assay
From: Cryptosporidium parvum rhomboid1 has an activity in microneme protein CpGP900 cleavage
Bait plasmid | Prey plasmid | Growth on selection plate | β-galactosidase activity |
|---|---|---|---|
pGBKT7-53 | pGADT7-T | + | + |
pGBKT7-CpROM1 | pGADT7-CpGP900 | + | + |
pGBKT7-CpROM4 | pGADT7-CpGP900 | – | – |
pGBKT7-CpROM1 | pGADT7-CpTRAP-C1 | – | – |
pGBKT7-CpROM4 | pGADT7-CpTRAP-C1 | – | – |
pGBKT7-TgROM2 | pGADT7-CpTRAP-C1 | + | + |
pGBKT7-Lam | pGADT7-T | – | – |