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Table 2 Interactions of CpROMs, TgROM2 and MIC proteins by yeast two-hybrid assay

From: Cryptosporidium parvum rhomboid1 has an activity in microneme protein CpGP900 cleavage

Bait plasmid Prey plasmid Growth on selection plate β-galactosidase activity
pGBKT7-53 pGADT7-T + +
pGBKT7-CpROM1 pGADT7-CpGP900 + +
pGBKT7-CpROM4 pGADT7-CpGP900
pGBKT7-CpROM1 pGADT7-CpTRAP-C1
pGBKT7-CpROM4 pGADT7-CpTRAP-C1
pGBKT7-TgROM2 pGADT7-CpTRAP-C1 + +
pGBKT7-Lam pGADT7-T
  1. +, growth and/or β-galactosidase activity; –, no growth and/or no β-galactosidase activity