Table 1 List of oligonucleotides
From: Stimulation and quantification of Babesia divergens gametocytogenesis
Gene name and sequence reference | Sequencing primers | qRT-PCR primers | ||
|---|---|---|---|---|
Sequence 5′-3′ and amplicon length (bp) | Sequence 5′-3′ and amplicon length (bp) | |||
gapdh (LK934710) | F: TTGACTGTCGATGGTGCTTC | 391 | F: TACTTACGAGCAGATCGTTGC | 140 |
R: ACCATGACACAAGCTTCACG | R: CGGCCTTGACATCGAAAATG | |||
actin (LK934710) | F: GCTTTGTTACATTGCCCTCG | 437 | F: GTCAGCGTATGACGAAGGAG | 131 |
R: CCTCCTTGGTGATCCACATC | R: CTGGAAGGTGGAAAGGGATG | |||
b-tubulin (LK934711) | F: TTCCCCAGACTGCACTTCTT | 400 | F: GAGTGGATCCCACACAACAC | 138 |
R: TGTGTACCAGTGAAGGAAGG | R: CATTGCTGTGAATTGCTCCG | |||
18S (FJ944825) | – | – | F: ATGCCTAGTATGCGCAAGTC | 131 |
– | – | R: AAGCCGACGAATCGGAAAG | ||
bdccp1 (FJ943575) | F: GATCGTTCCTCGCTAGCCTAT | 639 | F: CGCATGCCAGAAAAACAACC | 132 |
R: TGCACTGATTTACGCAGCTC | R: GCGTCTTTCAGACATCCTCG | |||
bdccp2 (FJ943576) | F: GCGGGAGAACATGTAGGATG | 701 | F: CTGTGAGGCCAACTACTGTG | 135 |
R: TTCGCAACACAGCTCACAAT | R: AAGTGGTCCACGGTTTTCTG | |||
bdccp3 (FJ943577) | F: CCCACCTCCTTTGACTTCAG R: GTGCATCTTGAGCACGAAAA | 780 | F: GTTGTGGTAAAAGCTGCATGG R: AGAATCGTGACAACTGCCTC | 139 |