Eco-epidemiology of porcine trypanosomosis in Karim Lamido, Nigeria: prevalence, seasonal distribution, tsetse density and infection rates

Table 1 Primer names, targets, sequences and amplicon sizes for PCR detection of African trypanosomes

Primer [Reference]	Target species	Target gene	Sequence (5'-3')	Amplicon size (bp)
TBR1 [44]	Trypanozoon	177 bp repeat	cgaatgaatattaaacaatgcgcagt	173
TBR2 [44]		Sequence	agaaccatttattagctttgttgc
TCK1 [45]	T. c. kilifi	SDNAm	gtgcccaaatttgaagtgat	294
TCK2 [45]			actcaaaatcgtgcacctcg
TCF1 [45]	T. c. forest	SDNAm	ggacacgccagaaggtactt	350
TCF2 [45]			gttctcgcaccaaatccaac
TCS1 [45]	T. c. savannah	SDNAm	cgagaacgggcactttgcga	316
TCS2 [45]			ggacaaagaaatcccgcaca
TVWI [46]	T. vivax	ILDat 1.2	gtgctccatgtgccacgttg	175
TVW2 [46]			catatggtctgggagcgggt
TeRoTat 920 F [47]	T. evansi	RoTat 1.2	ctgaagaggttggaaatggagaag	151
TeRoTat 1070R [47]		VSG gene	gtttcggtggttctgttgttgtta
TgsGP-F [48]	T. brucei	TgsGP	gctgctgtgttcggagagc	308
TgsGP-R [48]	gambiense		gccatcgtgcttgccgctc
SRA-F [49]	T. brucei	SRA	atagtgacaagatgcgtactcaacgc	284
SRA-R [49]	rhodesiense		aatgtgttcgagtacttcggtcacgct

ISSN: 1756-3305