Eco-epidemiology of porcine trypanosomosis in Karim Lamido, Nigeria: prevalence, seasonal distribution, tsetse density and infection rates

Table 2 Reaction conditions for different polymerase chain reactions

PCR	PD Temp/time	No. of cycles	DTemp/time	Tm Temp/time	ETemp/time	FE Temp/time
TBR	94 °C, 3 min	35	94 °C, 30 s	55 °C, 1 min	72 °C, 1 min	72 °C, 5 min
TCF, TCK and TCS	94 °C, 3 min	35	94 °C, 1 min	60 °C, 2 min	72 °C, 1 min	72 °C, 5 min
WA-TV	94 °C, 1 min	35	94 °C, 30 s	55 °C, 1 min	72 °C, 2 min	72 °C, 5 min
TeRoTat	95 °C, 2 min	35	95 °C, 30 s	58 °C, 30 s	72 °C, 1 min	72 °C, 5 min
TgsGP and SRA	95 °C, 15 min	45	95 °C, 1 min	63 °C, 1 min	72 °C, 1 min	72 °C, 5 min

Abbreviations: PD (Pre-denaturation), D (denaturation), Tm (annealing temperature), E (extension), FE (final extension), Temp (temperature)

ISSN: 1756-3305