Skip to main content

Table 2 Reaction conditions for different polymerase chain reactions

From: Eco-epidemiology of porcine trypanosomosis in Karim Lamido, Nigeria: prevalence, seasonal distribution, tsetse density and infection rates

PCR PD Temp/time No. of cycles DTemp/time Tm Temp/time ETemp/time FE Temp/time
TBR 94 °C, 3 min 35 94 °C, 30 s 55 °C, 1 min 72 °C, 1 min 72 °C, 5 min
TCF, TCK and TCS 94 °C, 3 min 35 94 °C, 1 min 60 °C, 2 min 72 °C, 1 min 72 °C, 5 min
WA-TV 94 °C, 1 min 35 94 °C, 30 s 55 °C, 1 min 72 °C, 2 min 72 °C, 5 min
TeRoTat 95 °C, 2 min 35 95 °C, 30 s 58 °C, 30 s 72 °C, 1 min 72 °C, 5 min
TgsGP and SRA 95 °C, 15 min 45 95 °C, 1 min 63 °C, 1 min 72 °C, 1 min 72 °C, 5 min
  1. Abbreviations: PD (Pre-denaturation), D (denaturation), Tm (annealing temperature), E (extension), FE (final extension), Temp (temperature)