Table 1 Primer pairs used for amplification of rickettsial genes
From: Rickettsia vini n. sp. (Rickettsiaceae) infecting the tick Ixodes arboricola (Acari: Ixodidae)
Target gene, primer pair no., primer name | Specifity | Sequence | Amplified fragment (nt) | Reference | |
|---|---|---|---|---|---|
gltA | Genus Rickettsia | ||||
1 | CS-78 | Â | 5'-GCAAGTATCGGTGAGGATGTAAT-3' | 401 | [19] |
| Â | CS-323 | Â | 5'-GCTTCCTTAAAATTCAATAAATCAGGAT-3' | Â | [19] |
2 | CS-239 | Â | 5'-GCTCTTCTCATCCTATGGCTATTAT-3' | 834 | [38] |
| Â | CS-1069 | Â | 5'-CAGGGTCTTCGTGCATTTCTT-3' | Â | [38] |
ompA | Spotted fever group (SFG) | ||||
3 | Rr190.70p | Â | 5'-ATGGCGAATATTTCTCCAAAA-3' | 632 | [39] |
| Â | 190-701 | Â | 5'-GTTCCGTTAATGGCAGCATCT-3' | Â | [40] |
ompB | Genus Rickettsia a | ||||
4 | 120-M59 | Â | 5'-CCGCAGGGTTGGTAACTGC-3' | 820 | [41] |
| Â | 120-807 | Â | 5'-CCTTTTAGATTACCGCCTAA-3' | Â | [41] |
htrA | Genus Rickettsia | ||||
5 | 17k-5 | Â | 5'-GCTTTACAAAATTCTAAAAACCATATA-3' | 549 | [38] |
| Â | 17k-3 | Â | 5'-TGTCTATCAATTCACAACTTGCC-3' | Â | [38] |