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Table 1 List of primers and probe for the lateral-flow stripe, real-time RPA assay and qPCR based on the Schistosoma japonicum genomic DNA

From: Recombinase polymerase amplification combined with a lateral flow dipstick for rapid and visual detection of Schistosoma japonicum

Assay format

Name

Sequence 5′–3′

Lateral-flow stripe RPA

RF

CCCAAGTCTCAGTGAAGTTGTGAAGGCTAT

RR

Biotin-GTTAGTGTTCGAGACCAGTCAGATGGGATT

Probe

FAM-CTTAAAGCGAGGGAGAGCGGCAGGACCAG

ATG[THF]ATTGACCCCTGAGATAT-ph

Real- time RPA

RF

CCCAAGTCTCAGTGAAGTTGTGAAGGCTAT

RR

GTTAGTGTTCGAGACCAGTCAGATGGGATT

Probe

CTTAAAGCGAGGGAGAGCGGCAGGACCAGA

[FAM-dT]G[THF]A[BHQ-dT]TGACCCCTGAGATAT-/C3-spacer/

qPCR

RF

GACAGGTTCTGGAACATAGG

RR

GGTCAATTCCGAAGACAATC

  1. Abbreviations: FAM 6-Carboxyfluorescein, THF tetrahydrofuran, BHQ black hole quencher
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Parasites & Vectors

ISSN: 1756-3305

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