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Fig. 3 | Parasites & Vectors

Fig. 3

From: The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis

Fig. 3

Characterization of Cathepsin L protein levels by IFA. Representative images of IFA of midguts and salivary glands of uninfected and A. phagocytophilum-infected adult female I. scapularis. Tick tissues were stained with rabbit anti-Cathepsin L (mature region No. pab0213-0; Covalab, Villeurbanne, France) antibodies (green, FITC) or DAPI (blue), and images were superimposed after staining (right panels). Preimmune control serum-treated samples showed similar results for uninfected and infected ticks. Uninfected and infected samples stained with anti-Cathepsin L antibodies showed higher protein levels in infected midguts while Cathepsin L was underrepresented in infected salivary glands when compared to uninfected controls (arrowheads). Scale-bars: 10 μm

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