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Table 3 Differential expression/representation of enzymes involved in tick hemoglobinolytic and heme transport pathways in response to A. phagocytophilum infection

From: The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis

ID

Description

Log2 (infected/uninfected) fold change (mRNA/protein)

Role in hemoglobin digestion

Midguts

Salivary glands

Tick hemoglobinolytic pathway

 EF428204

Cathepsin D

nf/ns

ns/nf

Primary cleavage

 A4GTA5

 HQ615697

Cathepsin D2

nf/ns

ns/nf

Primary cleavage

 E7E820

 ISCW000202

Legumain

-0.6/ns

ns/ns

Primary cleavage

 B7P6S9

 ISCW015983

Legumain

-1.3/nf

nf/nf

Primary cleavage

 B7P2C6

 ISCW000076

Cathepsin L

-7.3/nf

nf/nf

Primary and secondary cleavage

 B7P3N8

 JX502821

Cathepsin L

nf/nf

nf/-2.9

Primary and secondary cleavage

 J9QSA1

 ISCW000080

Cathepsin B

+0.5/ns

nf/-2.1

Secondary and tertiary cleavage

 B7P3P1

 EU551624

Cathepsin B

nf/ns

nf/-2.0

Secondary and tertiary cleavage

 B7SP39

 ISCW013346

Cathepsin B

-1.6/ns

ns/-2.5

Secondary and tertiary cleavage

 B7QCU7

 ISCW000078

Cathepsin B

+0.2/ns

-4.3/-1.8

Secondary and tertiary cleavage

 B7P3P0

 ISCW003494

Cathepsin C

+0.3/ns

-2.6/+2.2

Tertiary cleavage

 B7PEB4

 ISCW001779

Leucine aminopeptidase

+2.2/ns

ns/nf

Tertiary cleavage

 B7P2N4

 ISCW023735

Leucine aminopeptidase

+2.0/ns

+1.1/ns

Tertiary cleavage

 B7QLQ7

 ISCW001780

Leucine aminopeptidase

+1.4/ns

+0.5/ns

Tertiary cleavage

 B7P2N5

 ISCW013904

Serine carboxipeptidase

+1.0/ns

ns/ns

Tertiary cleavage

 B7QLB7

 ISCW024536

Serine carboxipeptidase

-0.5/ns

ns/nf

Tertiary cleavage

 B7Q049

 ISCW024751

Serine carboxipeptidase

-0.7/ns

ns/ns

Tertiary cleavage

 B7QD81

 ISCW024883

Serine carboxipeptidase

-4.5/nf

-2.4/nf

Tertiary cleavage

 B7QK83

 ISCW007492

Serine carboxipeptidase

-1.7/ns

ns/ns

Tertiary cleavage

 B7PTE5

 ISCW003059

Serine carboxipeptidase

-1.2/nf

ns/nf

Tertiary cleavage

 B7PC00

Tick heme transport pathway

 ISCW001847

Heme-responsive gene 1 (HRG1)

-0.8/nf

ns/nf

Heme transporter

 B7P8M4

 ISCW021709

Heme-binding lipoprotein (HELP)

+3.3/ns

-0.2/ns

Heme transporter

 B7Q406

 ISCW013727

Vitellogenin 1 (VG1)

ns/ns

ns/nf

Heme transporter

 B7QJ67

 ISCW021228

Vitellogenin 2 (VG2)

-1.1/nf

ns/nf

Heme transporter

 B7Q7E5

  1. Transcriptomics RNAseq and proteomics data from A. phagocytophilum-infected and uninfected tick samples were obtained from Ayllón et al. [12]. Except for Cathepsins D and D2, which were included to show that these proteins were identified in the proteomics analysis but were not significantly different between infected and uninfected samples, only genes/proteins with statistically significant differences in at least one of the analyses (transcriptomics or proteomics) and samples (midguts or salivary glands) were included. Abbreviations: ID, gene (GenBank; http://www.ncbi.nlm.nih.gov) and protein (Uniprot; http://www.uniprot.org) accession numbers
  2. +, upregulated/overrepresented genes/proteins in infected vs uninfected ticks; -, downregulated/underrepresented genes/proteins in infected vs uninfected ticks; nf, not found; ns, not significant